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|Section2= |Section3= |Section4= |Section7= |Section8= }} Bromopyrogallol red is frequently used in analytical chemistry as a reagent for spectrophometric analysis and as an complexometric indicator. ==Uses, behaviors, and properties== Bromopyrogallol red is commonly used in analytical chemistry as a spectrophotometric reagent and complexometric indicator. When metal ion complexation reactions are carried out in the presence of quaternary ammonium salts, the analytical possibilities with bromopyrogallol red are even greater. Doing this also increases the color contrast and sensitivity of many elements for analysis. In addition, bromopyrogallol red can also be widely used as a metallochromic indicator. Bromopyrogallol red has been used as a complexometric indicator for determination of Bi, Cd, Co, Mg, and Mn. Bromopyrogallol red is protonized in 1 M H2S4 solution and changes gradually to its ionized forms in alkaline solution. In its neutral form, bromopyrogallol red is bipolar ion, containing a protonized carbonyl group as well as a dissociated sulfonate group. Through testing at isobestic points, it has been determined that bromopyrogallol red is a stronger acid than Pyrogallol Red. In the presence of ammonia salts, the bathochromic shifts increase. The hydrophilic group on the ammonia salts also change the dissociation of the reagent to be used with bromopyrogallol red to a more acidic region.〔 Bromopyrogallol red is also a triphenylmethane indictor. An efficient purification can be obtained by chromatographic separation on a polyamide column prewashed with HCl. Purity can then be checked by thin layer chromatography on a microcrystalline cellulose plate using either a system of butanol-acetic acid-water, n-propanol - water, or methanol-water. Another use of Bromopyrogallol red is as an idicator for titrations with Cd (pH 9.3), Mg (pH 10), Mn (pH10), and lanthanidoes ( pH 7). It also commonly used in back-titrations with Co, Cu, Ga, Pb, and Th. It is usually used as a 0.05% solution in 50% ethanol when used as an indicator. In pharmacy, bromopyrogallol red serves to bind proteins together. It is used in 30-100 μmol/L concentrations. It has been used in the electrophoretic mobility shift assay and p50 and title comp preincubated in assay buffer, pH 7.5 (room temp., 5 min). 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Bromopyrogallol red」の詳細全文を読む スポンサード リンク
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